Use of fluorescence probes for detection of reactive nitrogen species: A review

• Type: Another Publication in an International Scientific Journal
• Date: 2006

Authors

Gomes, A (Author) Other; Fernandes, E (Author) FFUP; Lima, JLFC (Author) FFUP

Journal

Title: Journal of Fluorescence

Vol. 16

Pages: 119-139

ISSN: 1053-0509

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 127 Citations

Scopus - 131 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-Q41

DOI: 10.1007/s10895-005-0030-3

Abstract (EN): The biological and toxicological effects that have been attributed to reactive nitrogen species (RNS) are increasingly stirring the scientific inquisitiveness about the molecular mechanisms involved. However, RNS present some characteristics that complicate their detection, namely their short lifetime and the normal presence of a variety of endogenous compounds capable of reacting with these reactive species, when the studies are performed in biological matrices. The development of methodologies capable of circumvent these difficulties is thus of fundamental importance. Fluorescence probes are particularly important due to their high sensibility and usefulness in temporal and spatial monitoring of RNS, particularly in microanalysis conditions in biological media akin to cells or tissues. In the present review is given an account of the fluorescence probes that have been used for detection of nitric oxide ((NO)-N-center dot), peroxynitrite anion (ONOO-), as well as of some of its derivatives in biological and nonbiological media.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 21