Niclosamide quantification in methyl-beta-cyclodextrin after derivatization to aminoniclosamide

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Manuel Algarra (Author) Other; Bruno B Campos (Author) Other; Jose E Rodriguez Borges (Author) FCUP; Francisco G Sanchez (Author) Other; Juan M Lopez Romero (Author) Other; Joaquim Esteves G E da Silva (Author) FCUP

Journal

Title: Journal of Inclusion Phenomena and Molecular Recognition in Chemistry

Vol. 72

Pages: 89-94

ISSN: 0923-0750

Publisher: Kluwer Academic Publishers

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-002-DH1

DOI: 10.1007/s10847-011-9945-2

Abstract (EN): A fluorescence derivatization method for the quantification of the anthelmintic drug niclosamide in organized media (methyl-beta-cyclodextrin) is described. The derivatization reaction is based on a 3 h reduction reaction of the nitro group in niclosamide to an amino group by hydrogenation catalyzed by Pd/C in methanol:ethyl acetate (1:3). The aminoniclosamide exhibits a maximum of fluorescence at 431 nm (excitation at 280 nm) that is markedly enhanced when methyl-beta-cyclodextrin is present. The methodology was assessed by the analysis of pure and dosage forms. The method showed a linear range between 0 and 2200 mu g L-1 with a limit of detection of 45.7 nIe and a precision (relative standard deviation) of 4.1% in niclosamide. The effect of various interferents, such as niclosamide, fructose and 2-cyano-6-metoxybenzothiazole, in several tolerance ratios, was investigated respected to aminoniclosamide.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6