Characterization and Modulation of Glucose Uptake in a Human Blood-Brain Barrier Model

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Manuela Meireles (Author) FMUP; Fatima Martel (Author) FMUP; Joao Araujo (Author) Other; Celestino Santos Buelga (Author) Other; Susana Gonzalez Manzano (Author) Other; Montserrat Duenas (Author) Other; Victor de Freitas (Author) FCUP; Nuno Mateus (Author) FCUP; Conceicao Calhau (Author) FMUP; Ana Faria (Author) FCUP

Journal

Title: Journal of Membrane Biology

Vol. 246

Pages: 669-677

ISSN: 0022-2631

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 22 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-006-7JZ

DOI: 10.1007/s00232-013-9583-2

Abstract (EN): The blood-brain barrier (BBB) plays a key role in limiting and regulating glucose access to glial and neuronal cells. In this work glucose uptake on a human BBB cell model (the hCMEC/D3 cell line) was characterized. The influence of some hormones and diet components on glucose uptake was also studied. H-3-2-deoxy-d-glucose ([H-3]-DG) uptake for hCMEC/D3 cells was evaluated in the presence or absence of tested compounds. [H-3]-DG uptake was sodium- and energy-independent. [H-3]-DG uptake was regulated by Ca2+ and calmodulin but not by MAPK kinase pathways. PKC, PKA and protein tyrosine kinase also seem to be involved in glucose uptake modulation. Progesterone and estrone were found to decrease H-3-DG uptake. Catechin and epicatechin did not have any effect, but their methylated metabolites increased [H-3]-DG uptake. Quercetin and myricetin decreased [H-3]-DG uptake, and glucuronic acid-conjugated quercetin did not have any effect. These cells expressed GLUT1, GLUT3 and SGLT1 mRNA.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9