Ion chromatographic determination of iodide in urine and serum using a tubular ion-selective electrode based on a homogeneous crystalline membrane

• Type: Article in International Scientific Journal
• Date: 1997

Authors

Almeida, AA (Author) FFUP; Jun, X (Author) Other; Lima, JLFC (Author) FFUP

Journal

Title: Mikrochimica Acta

Vol. 127

Pages: 55-60

ISSN: 0026-3672

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 25 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-CRV

DOI: 10.1007/bf01243164

Abstract (EN): The use of a laboratory-made iodide ionselective electrode with tubular configuration and based on a crystalline membrane (AgI/Ag2S) as the detector for ion chromatographic determination of iodide in urine and serum is described. A C18 reversed-phase column was coated with N-cetylpyridinium chloride to prepare a low-exchange-capacity analytical column and with hexadecyltrimethylammonium bromide to prepare a concentrator pre-column. A 2.0 ml min(-1) flow rate of deionized water and 0.1 mol l(-1) KNO3 solution was used for the pre-concentration and for the chromatographic separation, respectively. For optimum performance of the detector a background level of iodide was added into the column effluent. A linear relationship (r = 0.9997) between tubular electrode potential (as peak height) and iodide concentration in the range 5-400 mu g l(-1) and a detection limit of 1.47 mu g l(-1) were obtained. The method shows good reproducibility for both peak height (2.2% RSD) and retention time (1.3% RSD). Recoveries on its application to the samples were 93.0-100.9% for urine and 91.4-106.0% for serum.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6