High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Joana P N Ribeiro (Author) Other; Luis M Magalhaes (Author) Other; Salette Reis (Author) FFUP; Jose L F C Lima (Author) FFUP; Marcela A Segundo (Author) FFUP

Journal

Title: Analytical Sciences

Vol. 27 No. 5

Pages: 483-488

ISSN: 0910-6340

Publisher: Japan Society for Analytical Chemistry

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 24 Citations

Pubmed / Medline

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences > Pharmacological sciences

Other information

Authenticus ID: P-002-RQC

DOI: 10.2116/analsci.27.483

Resumo (PT): High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a microformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h−1 (microplate method) or of 15 h−1 (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method. <br> <br> <a target="_blank" href="http://www.jstage.jst.go.jp/article/analsci/27/5/27_483/_article "> Texto integral </a> <br> <br>

Abstract (EN): High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a rnicroformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h(-1) (microplate method) or of 15 h(-1) (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6