Single reaction interface flow system for chemiluminescent monitoring of mannitol based on its hydroxyl radical scavenger activity

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Cristina I C Silvestre (Author) Other; Joao L M Santos (Author) FFUP; Jose L F C Lima (Author) FFUP; Elias A G Zagatto (Author) Other

Journal

Title: Talanta

Vol. 77 No. 2

Pages: 518-521

ISSN: 0039-9140

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 10 Citations

ISI Web of Science

Scopus - 11 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-T85

DOI: 10.1016/j.talanta.2008.03.021

Resumo (PT): A single reaction interface flow analysis (SIFA) system for the monitoring of mannitol in pharmaceutical formulations and human urine is presented. The developed approach takes advantage of the mannitol scavenger aptitude to inhibit the chemiluminescent reaction between luminol and myoglobin in the absence of H2O2. The SIFA system facilitated the fully automation of the developed methodology, allowing the in-line reproducible handling of chemical species with a very short lifetime as is the case of the hydroxyl radical generated in the abovementioned luminol/myoglobin reaction. The proposed methodology allowed the determination of mannitol concentrations between 25 mmol L−1 and 1 mol L−1, with good precision (R.S.D. < 4.7%, n = 3) and a sampling frequency of about 60 h−1. The procedure was applied to the determination of mannitol in pharmaceuticals and in human urine samples without any pretreatment process. The results obtained for pharmaceutical formulations were statistically comparable to those provided by the reference method (R.D. < 4.6%); recoveries values obtained in the analysis of spiked urine samples (between 94.9 and 105.3% of the added amount) were also satisfactory. <br> <br> Keywords: Single reaction interface flow analysis; Mannitol determination; Hydroxyl radical; Myoglobin; Chemiluminescence <br> <a target="_blank" href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THP-4S4JYKY-9&_user=2460038&_coverDate=12%2F15%2F2008&_rdoc=13&_fmt=high&_orig=browse&_srch=doc-info(%23toc%235288%232008%23999229997%23700527%23FLA%23display%23Volume)&_cdi=5288&_sort=d&_docanchor=&_ct=76&_acct=C000057398&_version=1&_urlVersion=0&_userid=2460038&md5=c3e6b65d880bde1c967c1e09d0403f8f"> Texto integral</a> <br> <br>

Abstract (EN): A single reaction interface flow analysis (SIFA) system for the monitoring of mannitol in pharmaceutical formulations and human urine is presented. The developed approach takes advantage of the mannitol scavenger aptitude to inhibit the chemiluminescent reaction between luminol and myoglobin in the absence of H(2)O(2). The SIFA system facilitated the fully automation of the developed methodology, allowing the in-line reproducible handling of chemical species with a very short lifetime as is the case of the hydroxyl radical generated in the abovementioned luminol/myoglobin reaction. The proposed methodology allowed the determination of mannitol concentrations between 25 mmol L(-1) and 1 mol L(-1), with good precision (R.S.D. < 4.7%, n = 3) and a sampling frequency of about 60 h(-1). The procedure was applied to the determination of mannitol in pharmaceuticals and in human urine samples Without any pretreatment process. The results obtained for pharmaceutical formulations were statistically comparable to those provided by the reference method (R.D. < 4.6%); recoveries values obtained in the analysis of spiked urine samples (between 94.9 and 105.3% of the added amount) were also satisfactory.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4