First report of non-coloured flavonoids in Echium plantagineum bee pollen: differentiation of isomers by liquid chromatography/ion trap mass spectrometry

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Federico Ferreres (Author) Other; David M. Pereira (Author) FFUP; Patrícia Valentão (Author) FFUP; Paula B. Andrade (Author) FFUP

Journal

Title: Rapid Communications in Mass Spectrometry

Vol. 24 No. 6

Pages: 801-806

ISSN: 0951-4198

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 24 Citations

ISI Web of Science

Scopus - 30 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Health sciences

CORDIS: Health sciences > Pharmacological sciences > Pharmacognosy

Other information

Authenticus ID: P-003-904

Resumo (PT): Apicultural products have been widely used in diet complements as well as in phytotherapy. Bee pollen from Echium plantagineum was analysed by high-performance liquid chromatography/photodiode-array detection coupled to ion trap mass spectrometry (HPLC-PAD-MSn) with an electrospray ionisation interface. The structures have been determined by the study of the ion mass fragmentation, which characterises the interglycosidic linkage in glycosylated flavonoids and differentiates positional isomers. Twelve non-coloured flavonoids were characterised, being kaempferol-3-O-neohesperidoside the major compound, besides others in trace amounts. These include quercetin, kaempferol and isorhamnetin glycosides, with several of them being isomers. Acetylated derivatives are also described. This is the first time that non-coloured flavonoids are reported from this pollen, with MS fragmentation proving to be most useful in the elucidation of isomeric structures <br> <br> <a target="_blank" href="http://onlinelibrary.wiley.com/doi/10.1002/rcm.4454/abstract"> Texto integral</a> <br> <br>

Abstract (EN): Apicultural products have been widely used in diet complements as well as in phytotherapy. Bee pollen from Echium plantagineum was analysed by high-performance liquid chromatography/photodiode-array detection coupled to ion trap mass spectrometry (HPLC-PAD-MSn) with an electrospray ionisation interface. The structures have been determined by the study of the ion mass fragmentation, which characterises the interglycosidic linkage in glycosylated flavonoids and differentiates positional isomers. Twelve non-coloured flavonoids were characterised, being kaempferol-3-O-neohesperidoside the major compound, besides others in trace amounts. These include quercetin, kaempferol and isorhamnetin glycosides, with several of them being isomers. Acetylated derivatives are also described. This is the first time that non-coloured flavonoids are reported from this pollen, with MS fragmentation proving to be most useful in the elucidation of isomeric structures <br> <br> <a target="_blank" href="http://onlinelibrary.wiley.com/doi/10.1002/rcm.4454/abstract">Full text</a> <br> <br>

Language: English

Type (Professor's evaluation): Scientific