Tomato (Lycopersicon esculentum) Seeds: New Flavonols and Cytotoxic Effect

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Federico Ferreres (Author) Other; Marcos Taveira (Author) Other; David M Pereira (Author) Other; Patricia Valentao (Author) FFUP; Paula B Andrade (Author) FFUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 58

Pages: 2854-2861

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 42 Citations

Scopus - 54 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-003-8AY

DOI: 10.1021/jf904015f

Resumo (PT): In this study, seeds of Lycopersicon esculentum Mill. were analyzed by HPLC/UV-PAD/MSn-ESI. Fourteen flavonoids were identified, including quercetin, kaempferol, and isorhamnetin derivatives, with 13 of them being reported for the first time in tomato seeds. The major identified compounds were quercetin-3-O-sophoroside, kaempferol-3-O-sophoroside, and isorhamnetin-3-O-sophoroside. A significant cell proliferation inhibition (>80%), against rat basophile leukemia (RBL-2H3) cell line, was observed with this extract (IC50 = 5980 μg/mL). For acetylcholinesterase inhibitory activity, a concentration-dependent effect was verified (IC20 = 2400 μg/mL). The same behavior was noted regarding antioxidant capacity, evaluated against DPPH (IC10 = 284 μg/mL), nitric oxide (IC25 = 396 μg/L), and superoxide radicals (IC25 = 3 μg/mL). <br> <br> Keywords (keywords): Lycopersicon esculentum; seeds; HPLC/UV-PAD/ESI-MSn; phenolic compounds; antiproliferative activity <br> <a target="_blank" href="http://pubs.acs.org/doi/abs/10.1021/jf904015f "> Texto integral</a> <br> <br>

Abstract (EN): In this study, seeds of Lycopersicon esculentum Mill. were analyzed by HPLC/UV-PAD/MS(n)-ESI. Fourteen flavonoids were identified, including quercetin, kaempferol, and isorhamnetin derivatives, with 13 of them being reported for the first time in tomato seeds. The major identified compounds were quercetin-3-O-sophoroside, kaempferol-3-O-sophoroside, and isorhamnetin-3-O-sophoroside. A significant cell proliferation inhibition (>80%), against rat basophile leukemia (RBL-2H3) cell line, was observed with this extract (IC(50) = 5980 mu g/mL). For acetylcholinesterase inhibitory activity, a concentration-dependent effect was verified (IC(20) = 2400 mu g/mL). The same behavior was noted regarding antioxidant capacity, evaluated against DPPH (IC(10) = 284 mu g/mL), nitric oxide (IC(25) = 396 mu g/L), and superoxide radicals (IC(25) = 3 mu g/mL).

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8