Human osteoblast response to silicon-substituted hydroxyapatite

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Botelho, CM (Author) Other; Brooks, RA (Author) Other; Best, SM (Author) Other; Lopes, MA (Author) FEUP; Santos, JD (Author) FEUP; Rushton, N (Author) Other; Bonfield, W (Author) Other

Journal

Title: Journal of Biomedical Materials Research - Part A

Vol. 79A

Pages: 723-730

ISSN: 1549-3296

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 99 Citations

ISI Web of Science

Scopus - 133 Citations

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

CORDIS: Technological sciences > Engineering > Biomedical enginnering ; Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-004-FRE

DOI: 10.1002/jbm.a.30806

Abstract (EN): Human osteoblasts were cultured on hydroxyapatite (HA), 0.8 wt % silicon substituted hydroxyapatite (Si-HA) and 1.5 wt % Si-HA discs. The influence of these substrates on cell behaviour in vitro was assessed by measuring total protein in the cell lysate and the production of several phenotypic markers: collagen type I (COL 1), alkaline phosphatase (ALP), osteocalcin (OC), and the formation of bone mineral. After 7 days, beta-glycerophosphate and physiological levels of hydrocortisone were added to the culture medium to stimulate cell differentiation and mineral production. There was a significantly higher production of ALP on 1.5 wt % Si-HA at day 7 following which, the addition of hydrocortisone promoted the differentiation of cells on the other two substrates. Hydrocortisone addition also decreased the production of OC. During the period, when hydrocortisone was present, no significant difference in ever, following removal of hydrocortisone, cells responded to 0.8 wt % Si-HA with a significant increase in protein production. Using fluorescence microscopy, nodular structures labeled with tetracycline were observed on the surface of all substrates after 21 days. These structures were deposited on areas of high cell density but were not related to the presence or level of silicon in the substrate. These results indicate that human osteoblasts are affected by the presence of silicon in the HA substrate and that the timing of these effects may be dependent upon the level of silicon substitution. (c) 2006 Wiley Periodicals, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8