8-Amide and 8-carbamate substitution patterns as modulators of 7-hydroxy-4-methylcoumarin?s antidepressant profile: Synthesis, biological evaluation and docking studies

• Type: Article in International Scientific Journal
• Date: 2023

Authors

Matos, MJ (Author) FCUP; Novo, P (Author) Other; Mayan, L (Author) Other; Torres, I (Author) Other; Uriarte, E (Author) Other; Yanez, M (Author) Other; Fontenla, JA (Author) Other; Ortuso, F (Author) Other; Alcaro, S (Author) Other; Procopio, F (Author) Other; Rodriguez Franco, MI (Author) Other; Val, C (Author) Other; Loza, MI (Author) Other; Brea, J (Author) Other; Fernanda Borges (Author) FCUP; Vina, D (Author) Other

Journal

Title: European Journal of Medicinal Chemistry

Vol. 248

ISSN: 0223-5234

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 3 Citations

Other information

Authenticus ID: P-00X-RJB

DOI: 10.1016/j.ejmech.2023.115091

Abstract (EN): Psychiatric and neurological disorders affect millions of people worldwide. Currently available treatments may help to improve symptoms, but they cannot cure the diseases. Therefore, there is an urgent need for potent and safe therapeutic solutions. 8-Amide and 8-carbamatecoumarins were synthetized and evaluated as human monoamine oxidase A and B (hMAO-A and hMAO-B) inhibitors. Comparison between both scaffolds has been established, and we hypothesized that the introduction of different substituents can modulate hMAO activity and selectivity. N-(7-Hydroxy-4-methylcoumarin-8-yl)-4-methylbenzamide (9) and ethyl N-(7-hydroxy-4-methylcoumarin-8-yl)carbamate (20) proved to be the most active and selective hMAO-A inhibitors (IC50 = 15.0 nM and IC50 = 22.0 nM, respectively), being compound 9 an irreversible hMAO-A inhibitor twenty-four times more active in vitro than moclobemide, a drug used in the treatment of depression and anxiety. Based on PAMPA assay results, both compounds proved to be good candidates to cross the blood-brain barrier. In addition, these compounds showed non-significant cytotoxicity on neuronal viability assays. Also, the best compound proved to have a t1/2 of 6.84 min, an intrinsic clearance of 195.63 mu L min-1 mg-1 protein, and to be chemically stable at pH 3.0, 7.4 and 10.0. Docking studies were performed to better understand the binding affinities and selectivity profiles for both hMAO isoforms. Finally, theoretical drug-like properties calculations corroborate the potential of both scaffolds on the search for new therapeutic solutions for psychiatric disorders as depression.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11