HA and double-layer HA-P2O5/CaO glass coatings: influence of chemical composition on human bone marrow cells osteoblastic behavior

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Ferraz, MP (Author) Other; Fernandes, MH (Author) FMDUP; Santos, JD (Author) FEUP; Monteiro, FJ (Author) FEUP

Journal

Title: Journal of Materials Science: Materials in Medicine

Vol. 12 No. 9

Pages: 629-638

ISSN: 0957-4530

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 22 Citations

ISI Web of Science

Scopus - 25 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

CORDIS: Technological sciences > Engineering > Biomaterial engineering ; Technological sciences > Engineering > Biomedical enginnering

Other information

Authenticus ID: P-000-XD3

DOI: 10.1023/a:1011245828046

Abstract (EN): Human osteoblastic bone marrow derived cells were cultured for 28 days onto the surface of a glass reinforced hydroxyapatite (HA) composite and a commercial type HA plasma sprayed coatings, both in the "as-received" condition and after an immersion treatment with culture medium during 21 days. Cell proliferation and differentiation were analyzed as a function of the chemical composition of the coatings and the immersion treatment. Cell attachment, growth and differentiation of osteoblastic bone marrow cells seeded onto "as-received" plasma sprayed coatings were strongly affected by the time-dependent variation of the surface structure occurring during the first hours of culture. Initial interactions leading to higher amounts of adsorbed protein and zeta potential shifts towards negative charges appeared to result in surface structures with better biological performance. Cultures grown onto the pretreated coatings showed higher rate of cell proliferation and increased functional activity, as compared to those grown onto the corresponding "as-received" materials. However, the cell behavior was similar in the glass composite and HA coatings. The results showed that the glass composites present better characteristics for bone cell growth and function than HA. In addition, this work also provide evidence that the biological performance of the glass composites can be modulated and improved by manipulations in the chemical composition, namely in the content of glass added to HA. (C) 2001 Kluwer Academic Publishers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10