Visible-light photoactivated proanthocyanidin and kappa-carrageenan coating with anti-adhesive properties against clinically relevant bacteria

• Type: Article in International Scientific Journal
• Date: 2024

Authors

Santinon, C (Author) Other; Anabela Borges (Author) FEUP; Simões, M (Author) Other; Gonçalves, SC (Author) Other; Beppu, MM (Author) Other; Vieira, MGA (Author) Other; Simões M (Author) FEUP

Journal

Title: International Journal of Biological Macromolecules

Vol. 263

ISSN: 0141-8130

Publisher: Elsevier

Indexing

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Other information

Authenticus ID: P-010-4YH

DOI: 10.1016/j.ijbiomac.2024.130611

Abstract (EN): The increase of bacterial resistance to antibiotics is a growing concern worldwide and the search for new therapies could cost billions of dollars and countless lives. Inert surfaces are major sources of contamination due to easier adhesion and formation of bacterial biofilms, hindering the disinfection process. Therefore, the objective of this study was to develop a photoactivatable and anti-adhesive kappa-carrageenan coating using proanthocyanidin as a photosensitizer. The complete reduction (>5-log(10) CFU/cm(3)) of culturable cells of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa pathogens was achieved after 30 min of exposure to visible light (420 nm; 30 mW/cm(2)) with 5 % (w/v) of the photosensitizer. Cell membrane damage was confirmed by measuring potassium leakage, epifluorescence microscopy and bacterial motility analysis. Overall, visible light irradiation on coated solid surfaces mediated by proanthocyanidin showed no cytotoxicity and inactivated clinically important pathogens through the generation of reactive oxygen species, inhibiting bacterial initial adhesion. The developed coating is a promising alternative for a wide range of applications related to surface disinfection and food biopreservation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 15