Extraction, purification and characterization of galactomannans from non-traditional sources

• Type: Article in International Scientific Journal
• Date: 2009

Authors

Miguel A. Cerqueira (Author) Other; Ana C. Pinheiro (Author) Other; Bartolomeu W. S. Souza (Author) Other; Álvaro M. P. Lima (Author) Other; Clara Ribeiro (Author) Other; Cândida Miranda (Author) Other; José A. Teixeira (Author) Other; Renato A. Moreira (Author) Other; Manuel A. Coimbra (Author) Other; M. Pilar Gonçalves (Author) FEUP; António A. Vicente (Author) Other

Journal

Title: Carbohydrate Polymers

Vol. 75

Pages: 408-414

ISSN: 0144-8617

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 119 Citations

ISI Web of Science

Scopus - 131 Citations

Scientific classification

FOS: Engineering and technology > Other engineering and technologies

CORDIS: Physical sciences

Other information

Authenticus ID: P-003-N6X

DOI: 10.1016/j.carbpol.2008.07.036

Abstract (EN): This work presents a methodology for the extraction of galactomannans from seeds of four different species of Leguminosae (Adenanthera pavonina, Caesalpinia pulcherrima, Gleditsia triacanthos and Sophora japonica) to be used e.g. in the food and biomedical industries. The galactomannans were obtained by aqueous extraction followed by a precipitation with ethanol. This methodology is simpler and easier to perform than other existing extraction and purification methodologies, and because it avoids the use of organic solvents (other than ethanol), it is able to generate food grade substances and is environmentally friendlier. The yield of extraction in different stages of the process, monosaccharide composition, as well as physical and chemical parameters of the isolated galactomannans were determined and compared with previously published results. The mannose/galactose ratio of the extracted galactomannans ranged from 1.35 (A. pavonina) to 5.75 (S. japonica). The intrinsic viscosity ranged from 11.34 dL/g (C. pulcherrima) to 8.74 dL/g (S. japonica), while the viscosity average molecular mass ranged between 1.81 × 106 Da and 1.17 × 106 Da (A. pavonina > C. pulcherrima > G. triacanthos > S. japonica). The results confirm the suitability of the extraction and purification procedure to obtain galactomannans from non-traditional sources.

Language: English

Type (Professor's evaluation): Scientific