Ocular Cell Lines and Genotoxicity Assessment

• Type: Another Publication in an International Scientific Journal
• Date: 2020

Authors

Souto, EB (Author) Other; Campos, JR (Author) Other; Da Ana, R (Author) Other; Martins Gomes, C (Author) Other; Silva, AM (Author) Other; Souto, SB (Author) FMUP; Lucarini, M (Author) Other; Durazzo, A (Author) Other; Santini, A (Author) Other

Journal

Title: International Journal of Environmental Research and Public Health

Vol. 17 No. 9

Final page: 2046

ISSN: 1661-7827

Publisher: MDPI

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 4 Citations

Scientific classification

CORDIS: Natural sciences

Other information

Authenticus ID: P-00R-XMN

DOI: 10.3390/ijerph17062046

Abstract (EN): Genotoxicity screening tests aim to evaluate if and to what extent a compound in contact with the human body (e.g., a drug molecule, a compound from the environment) interacts with DNA. The comet assay is a sensitive method used to predict the risk of DNA damage in individual cells, as it quantifies the tape breaks, being the alkaline version (pH > 13) the most commonly used in the laboratory. Epithelial cells serve as biomatrices in genotoxicity assessments. As ca. 80% of solid cancers are of epithelial origin, the quantification of the DNA damage upon exposure of epithelial cells to a drug or drug formulation becomes relevant. Comet assays run in epithelial cells also have clinical applications in human biomonitoring, which assesses whether and to what extent is the human body exposed to environmental genotoxic compounds and how such exposure changes over time. Ocular mucosa is particularly exposed to environmental assaults. This review summarizes the published data on the genotoxicity assessment in estimating DNA damage in epithelial cells with a special focus on ocular cell lines. General comet assay procedures for ex vivo and in vivo epithelium samples are also described.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 17