In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Shirosaki, Y (Author) Other; Tsuru, K (Author) Other; Hayakawa, S (Author) Other; Osaka, A (Author) Other; Lopes, MA (Author) FEUP; Santos, JD (Author) FEUP; Fernandes, MH (Author) FMDUP

Journal

Title: Biomaterials

Vol. 26 No. 5

Pages: 485-493

ISSN: 0142-9612

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 119 Citations

ISI Web of Science

Scopus - 144 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology > Environmental biotechnology

CORDIS: Technological sciences > Engineering > Biomedical enginnering ; Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-000-4SJ

DOI: 10.1016/j.biomaterials.2004.02.056

Abstract (EN): Chitosan-silicate hybrids were synthesized using gamma-glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and Si-29 CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the Surface of a chitosan-GPSM hybrid without calcium were similar to those on a control Culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9