Reduced Fluoresceinamine as a Fluorescent Sensor for Nitric Oxide

• Type: Article in International Scientific Journal
• Date: 2010

Authors

duarte, aj (Author) Other; da silva, jcge (Author) FCUP

Journal

Title: Sensors

Vol. 10 No. 3

Pages: 1661-1669

ISSN: 1424-3210

Publisher: MDPI

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 13 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Physical sciences > Chemistry > Inorganic chemistry ; Physical sciences > Chemistry > Analytical chemistry > Spectrochemistry

Other information

Authenticus ID: P-003-910

DOI: 10.3390/s100301661

Abstract (EN): A new fluorescent sensor for nitric oxide (NO) is presented that is based on its reaction with a non fluorescent substance, reduced fluoresceinamine, producing the highly fluorescent fluoresceinamine. Using a portable homemade stabilized light source consisting of 450 nm LED and fiber optics to guide the light, the sensor responds linearly within seconds in the NO concentration range between about 10-750 mu M with a limit of detection (LOD) of about 1 mu M. The system generated precise intensity readings, with a relative standard deviation of less than 1%. The suitability of the sensor was assessed by monitoring the NO generated by either the nitrous acid decomposition reaction or from a NO-releasing compound. Using relatively high incubation times, the sensor also responds quantitatively to hydrogen peroxide and potassium superoxide, however, using transient signal measurements results in no interfering species.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9