Xanthohumol inhibits cell proliferation and induces apoptosis in human thyroid cells

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Carvalho, DO (Author) FCUP; Freitas, J (Author) Other; Nogueira, P (Author) Other; Henriques, SN (Author) Other; Carmo, AM (Author) Other; Castro, MA (Author) Other; Luis F Guido (Author) FCUP

Journal

Title: Food and Chemical Toxicology

Vol. 121

Pages: 450-457

ISSN: 0278-6915

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 15 Citations

Other information

Authenticus ID: P-00P-NSA

DOI: 10.1016/j.fct.2018.09.021

Abstract (EN): The cell growth inhibitory potential of xanthohumol (XN), a natural prenylflavonoid present in hops and beer, on human papillary thyroid cancer cells is reported. We demonstrate that XN decreases the proliferation of TPC-1 cancer cells in a dose and time dependent manners. At low concentration (10 mu M) XN was shown to significantly inhibit carcinogenesis by a mechanism that stops or slows down cell division, preserving the viability of the cells. At higher concentration (100 mu M) a decrease of cell viability was observed by induction of apoptosis. As evidenced, XN induced DNA fragmentation in TPC-1 cells and promoted cell cycle arrest, which decreased the percentage of cells in G1 phase and increased in S phase after 72 h of treatment. Furthermore, XN exposure triggered an increase in caspase-3 and caspase-7 activity, supporting its role in the activation of apoptosis. Cell free studies demonstrated that high concentrations of XN are responsible for an increase of free radicals generated in a Fenton system which may mediate apoptosis through a pro-oxidant pathway. Altogether, our data show that XN induces the apoptosis of TPC-1 cancer cells in a concentration-dependent manner, suggesting XN to be a promising candidate for thyroid cancer therapy.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8