Pseudomonas fluorescens tolerance to benzyldimethyldodecyl ammonium chloride: altered phenotype and cross-resistance

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Maria Sousa Silva (Author) Other; Manuel Simões (Author) FEUP; Luís Melo (Author) FEUP; Idalina Machado (Author) FEUP

Journal

Title: Journal of Global Antimicrobial Resistance

Vol. 15

Pages: 188-195

ISSN: 2213-7165

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

ISI Web of Science

Scopus - 3 Citations

Associated Projects

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

POMACEA - Tecnologias económicas para controlo de (bio)fouling em membranas através da otimização de métodos de pré-tratamentos de limpeza

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Other information

Authenticus ID: P-00P-SCP

DOI: 10.1016/j.jgar.2018.07.004

Abstract (EN): Objectives: Benzyldimethyldodecyl ammonium chloride (BDMDAC) is a quaternary ammonium compound (QAC) with bactericidal action that is used as an active molecule in detergent formulations. Pseudomonas fluorescens is a Gram-negative bacterium with versatile metabolism that is frequently present in biofilms on industrial surfaces. This work reports P. fluorescens adaptation to BDMDAC and subsequent concurrent reduced susceptibility to the QAC benzalkonium chloride (BAC) and the antimicrobial ciprofloxacin (CIP). Methods: Stepwise adaptation to increasing concentrations of BDMDAC was easily achieved and caused changes in the bacterial phenotype of P. fluorescens. Adaptation was evaluated through minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination and was subsequently confirmed by time-kill curves. Biofilm phenotype (biomass and number of cells) was characterised for the adapted and reference strains after treatment with BDMDAC, BAC and CIP. Results: Susceptibility to BAC and CIP was reduced in adapted P. fluorescens. Biofilms developed by the adapted strain had 20% more mass and a higher number of bacteria (2 log). Conclusions: This study revealed that exposure to sublethal concentrations of BDMDAC may select tolerant strains to that product as well as to related products and unrelated antimicrobial agents. (C) 2018 International Society for Chemotherapy of Infection and Cancer.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8