Oxidative stress and genotoxicity of an organic and an inorganic nanomaterial to Eisenia andrei: SDS/DDAB nano -vesicles and titanium silicon oxide

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Correia, B (Author) Other; Lourenco, J (Author) Other; Marques, S (Author) Other; Nogueira, V (Author) Other; Gavina, A (Author) Other; Rasteiro, MD (Author) Other; Antunes, F (Author) Other; Mendo, S (Author) Other; Ruth Pereira (Author) FCUP

Journal

Title: Ecotoxicology and Environmental Safety

Vol. 140

Pages: 198-205

ISSN: 0147-6513

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 9 Citations

Other information

Authenticus ID: P-00M-GVF

DOI: 10.1016/j.ecoenv.2017.02.035

Abstract (EN): In the past few years the number of studies on the toxic effects of nanomaterials (NMs) in the environment increased significantly. Nonetheless, the data is still scarce, since there is a large number of NMs and new ones are being developed each day. Soils are extremely important for life, and are easily exposed to the released NMs, thus enhanced efforts are needed to study the impacts on soil biota. The objective of the present work was to determine if different concentrations of two NMs, one inorganic (TiSiO4) and other organic (nano-vesicles of sodium sodecyl sulfate/didodecyl dimethylammonium bromide - SDS/DDAB), are genotoxic to soil invertebrates. Additionally, it was intended to understand whether, in the event of occurring, genotoxicity was caused by the incapability of the cells to deal with the oxidative stress caused by these NMs. With that purpose, Eisenia andrei were exposed for 30 days to the artificial OECD soil contaminated with different concentrations of the NMs being tested. After the exposure, coelomocytes were extracted from earthworms and DNA damage was measured by the comet assay. The activity of antioxidant enzymes (e.g. glutathione peroxidase, glutathione reductase and glutathione-S-Transferase) and lipid peroxidation were also assessed. The results showed that both NMs were genotoxic, particularly TiSiO4 for which significant DNA damages were recorded for concentrations above 444 mg of TiSiO4-NM/kg of soil(dw). Since no statistically significant differences were found in the tested antioxidant enzymes and in lipid peroxidation, the mechanism of genotoxicity of these NMs seemed to be unrelated with oxidative stress.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8