Protein imprinted materials designed with charged binding sites on screen-printed electrode for microseminoprotein-beta determination in biological samples

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Rebelo, TSCR (Author) Other; Carlos M Pereira (Author) FCUP; Sales, MGF (Author) Other; Noronha, JP (Author) Other; Silva, F (Author) FCUP

Journal

Title: Sensors and Actuators, B: Chemical

Vol. 223

Pages: 846-852

ISSN: 0925-4005

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-00G-SCJ

DOI: 10.1016/j.snb.2015.09.133

Abstract (EN): In the past few years a large effort is being made aiming at the development of fast and reliable tests for cancer biomarkers. Protein imprinted sensors can be a fast and reliable strategy to develop tailor made sensors for a large number of relevant molecules. This work aims to produce, optimize and use in biological samples a biosensor for microseminoproteinbeta (MSMB). Caffeic acid (CAF) electropolimerization was performed in the presence of microseminoprotein-beta (MSMB) creating target protein specific cavities on the surface of a screen-printed carbon. Dopamine was introduced as charged monomer labelling the binding site and was allowed to self-organize around the protein. The subsequent electropolimerization was made by applying a constant potential of +2.0 V, for 30s, on a carbon screen-printed electrode, immersed in a solution of protein and CAF prepared in phosphate buffer. The sensor with charged monomers showed a more sensitive response, with an average slope of -7.59 mu A/decade, linear concentration range of 0.5-100 ng/mL and a detection limit of 0.12 ng/mL. The corresponding non-imprinted sensor displayed an inconsistent response over the range of the calibration curve. The biosensor was successfully applied to the analysis of MSMB in serum and urine samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7