Biofilm Localization in the Vertical Wall of Shaking 96-Well Plates

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Luciana C. Gomes (Author) FEUP; Joana M. R. Moreira (Author) FEUP; Manuel Simões (Author) FEUP; Luís F. Melo (Author) FEUP; Filipe J. Mergulhão (Author) FEUP

Journal

Title: Scientifica

Vol. 2014

Pages: 1-6

Publisher: Hindawi

Indexing

ISI Web of Science

Scopus - 21 Citations

Associated Projects

SYNBIOBACTHER - Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética

Filmstart - Estudo das condições iniciais que controlam a formação de biofilmes

Other information

Authenticus ID: P-00K-WJ0

DOI: 10.1155/2014/231083

Abstract (EN): Microtiter plates with 96 wells are being increasingly used for biofilm studies due to their high throughput, low cost, easy handling, and easy application of several analytical methods to evaluate different biofilm parameters.These methods provide bulk information about the biofilm formed in each well but lack in detail, namely, regarding the spatial location of the biofilms. This location can be obtained by microscopy observation using optical and electron microscopes, but these techniques have lower throughput and higher cost and are subjected to equipment availability. This work describes a differential crystal violet (CV) staining method that enabled the determination of the spatial location of Escherichia coli biofilms formed in the vertical wall of shaking 96-well plates. It was shown that the biofilms were unevenly distributed on the wall with denser cell accumulation near the air-liquid interface. The results were corroborated by scanning electron microscopy and a correlation was found between biofilm accumulation and the wall shear strain rates determined by computational fluid dynamics. The developed method is quicker and less expensive and has a higher throughput than the existing methods available for spatial location of biofilms in microtiter plates.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7