Combinatorial Activity of Flavonoids with Antibiotics Against Drug-Resistant Staphylococcus aureus

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Ana Cristina Abreu (Author) FEUP; Sofia C. Serra (Author) Other; Anabela Borges (Author) FEUP; Maria José Saavedra (Author) Other; Andrew J. Mcbain (Author) Other; António J. Salgado (Author) Other; Manuel Simões (Author) FEUP

Journal

Title: Microbial Drug Resistance

Vol. 21 No. 6

Pages: 600-609

ISSN: 1076-6294

Publisher: Mary Ann Liebert

Indexing

ISI Web of Knowledge - 6 Citations

ISI Web of Science

Scopus - 17 Citations

Scientific classification

FOS: Engineering and technology

CORDIS: Physical sciences > Chemistry

Associated Projects

Fitodesinfetantes - Desinfeção de bactérias planctónicas e biofilmes em ambientes hospitalares com produtos fitoquímicos

Bioresist - Estudo da influência do fenótipo de biofilmes na sua resiliência e resistência

Other information

Authenticus ID: P-00J-Y5J

DOI: 10.1089/mdr.2014.0252

Abstract (EN): The use of resistance-modifying agents is a potential strategy that is used to prolong the effective life of antibiotics in the face of increasing antibiotic resistance. Since certain flavonoids are potent bacterial efflux pump inhibitors, we assessed morin, rutin, quercetin, hesperidin, and (+)-catechin for their combined activity with the antibiotics ciprofloxacin, tetracycline, erythromycin, oxacillin, and ampicillin against drug-resistant strains of Staphylococcus aureus, including methicillin-resistant S. aureus. Four established methods were used to determine the combined efficacy of each combination: microdilution checkerboard assays, time-kill determinations, the Etest, and dual disc-diffusion methods. The cytotoxicity of the flavonoids was additionally evaluated in a mouse fibroblast cell line. Quercetin and its isomer morin decreased by 3- to 16-fold the minimal inhibitory concentration of ciprofloxacin, tetracycline, and erythromycin against some S. aureus strains. Rutin, hesperidin, and (+)-catechin did not promote any potentiation of antibiotics. Despite the potential cytotoxicity of these phytochemicals at a high concentration (fibroblast IC50 of 41.8 and 67.5mg/L, respectively), quercetin is commonly used as a supplement for several therapeutic purposes. All the methods, with exception of the time-kill assay, presented a high degree of congruence without any apparent strain specificity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10