Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals

• Type: Article in International Scientific Journal
• Date: 2015

Authors

Mota, R (Author) Other; Pereira, SB (Author) Other; Meazzini, M (Author) Other; Fernandes, R (Author) Other; Santos, A (Author) FCUP; Evans, CA (Author) Other; De Philippis, R (Author) Other; Wright, PC (Author) Other; Tamagnini, P (Author) FCUP

Journal

Title: Data in Brief

Vol. 4

Pages: 152-158

ISSN: 2352-3409

Publisher: Elsevier

Indexing

ISI Web of Knowledge

Scopus - 3 Citations

Other information

Authenticus ID: P-00G-BT8

DOI: 10.1016/j.dib.2015.04.015

Abstract (EN): The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110, grown in medium supplemented with an essential metal (Cu2+) or a non-essential metal (Cd2+),were compared using iTRAQ technology. The data were obtained within a larger study that evaluated the overall effects of different heavy metals on growth/survival, EPS production and ultrastructure of this cyanobacterium [1]. To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece's proteomes were evaluated after chronic and acute exposure to Cu2+ and Cd2+ in two independent 8-plex iTRAQ studies. For the chronic exposure 0.1mg/l of Cu2+ or 5mg/l of Cd2+ were used for 10 and 20 days, while in the acute experiments the cells were exposed to 10× these concentrations for 24h. 202 and 268 proteins were identified and quantified for studies 1 (Cu2+) and 2 (Cd2+), respectively. The majority of the proteins with significant fold changes were associated with photosynthesis, CO<inf>2</inf> fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolism. © 2015 The Authors.

Language: English

Type (Professor's evaluation): Scientific