Validation of respirometry as a short-term method to assess the efficacy of biocides

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Simoes, M (Author) Other; Pereira, MO (Author) Other; Vieira, MJ (Author) Other

Journal

Title: Biofouling

Vol. 21

Pages: 9-17

ISSN: 0892-7014

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 33 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-4SG

DOI: 10.1080/08927010500066982

Abstract (EN): This study shows that a short-term respirometric measurement based on the rate of oxygen uptake needed to oxidize glucose is a reliable and fast method to assess biocide efficacy against P. fluorescens cells. Respiratory activity using oxygen consumption rate, the determination of viable and nonviable cells using Live/ Dead (R) BacLight (TM) kit and colony formation units (CFU), were compared as indicators of the biocidal efficacy of ortho-phthalaldehyde (OPA). The results showed that determining the effect of OPA against P. fluorescens using the different methods leads to different conclusions. The minimum bactericidal concentration (MBC) was 80 mg l(-1), 100 mg l(-1) and 65 mg l(-1) respectively, using respiratory activity, viability using BacLight counts and culturability. The plate count method was shown to underestimate the biocidal action of OPA, whilst data from respirometry and viability using Live/ Dead BacLight kit correlated strongly and were not statistically different when yellow cells were considered nonviable. Respirometry therefore represents an expeditious, non-destructive and accurate method to determine the antimicrobial action of biocides against aerobic heterotrophic bacteria.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9