Development of headspace solid-phase microextraction-gas chromatography-mass spectrometry methodology for analysis of terpenoids in Madeira wines

• Type: Another Publication in an International Scientific Journal
• Date: 2006

Authors

Camara, JS (Author) Other; Alves, MA (Author) FEUP; Marques, JC (Author) Other

Journal

Title: Analytica Chimica Acta

Vol. 555 No. 2

Pages: 191-200

ISSN: 0003-2670

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 82 Citations

Scopus - 106 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-NPM

DOI: 10.1016/j.aca.2005.09.001

Abstract (EN): A dynamic headspace solid-phase microextraction methodology was developed for analysis of varietal aroma compounds in must and Madeira wine samples, a spirit wine with an ethanol content of 18% (v/v). The factors with influence in the headspace solid-phase microextraction efficiency such as: fibre coating, extraction time and temperature, pH, ionic strength, ethanol content, desorption time and temperature, were optimised and the method validated. The best results were obtained for a 85 Run polyacrylate fibre, with a 60 min headspace for must and 120 min for wine samples, in a 2.4 ml sample at 40 degrees C with 30% of NaCl. The extract is injected in the splitless mode in a GC-MS Varian system, Saturn III, and separated on a Stabilwax capillary column. The linear dynamic range of the method covers the normal range of occurrence of analytes in wine with typical r(2) between 0.985 (beta-ionone) and 0.998 (linalool) for musts and between 0.980 (alpha-terpineol) and 0.999 (linalool) for must and wine samples, respectively. For must samples the reproducibility ranges from 2.5% (citronellol) to 14.4% (nerolidol) (as R.S.D.), and from 4.8% (citronellol) to 14.2% (neiolidol) for wine samples. The analysis of spiked samples has shown that matrix effects do not significantly affect method performance. Limits of detection obtained are in low mu g I-1 range for all compounds analysed in this study.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10