Glutamate sol-gel amperometric biosensor based on co-immobilised NADP(+) and glutamate dehydrogenase

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Gomes, SP (Author) Other; Dolezalova, J (Author) Other; Araujo, AN (Author) FFUP; Couto, CMCM (Author) Other; Montenegro, MCBSM (Author) FFUP

Journal

Title: Journal of Analytical Chemistry

Vol. 68

Pages: 794-800

ISSN: 1061-9348

Publisher: Pleiades Publishing

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 3 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-006-7VZ

DOI: 10.1134/s1061934813090049

Abstract (EN): In this work the preparation and electrochemical characterisation of a new glutamate amperometric sol-gel biosensor is described. A carbon paste electrode was electrochemically modified with the phenothiazine dye methylene green (MG). The NADP(+) and glutamate dehydrogenase were co-immobilised in a sol-gel matrix. When coupled to a flow injection system (FIA) the biosensor showed good electrocatalytic activity towards NADPH oxidation at the potential of + 0.3 V vs. Ag/AgCl; KCl sat., which represents a strong overpotential reduction. The biosensor yielded a linear response from 5.0 x 10(-5) to 1.0 x 10(-2) M glutamate concentration, with a detection limit of 5 x 10(-6) M and reproducibility of results better than 2.3% (RSD). Moreover, the implemented biosensor showed to be still useful after 7 months, 500 determinations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7