Separation Region and Strategies for Proteins Separation by Salt Gradient Ion-Exchange SMB

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Ping Li (Author) Other; Jianguo Yu (Author) Other; Guohua Xiu (Author) Other; Alírio E. Rodrigues (Author) FEUP

Journal

Title: Separation Science and Technology

Vol. 43 No. 1

Pages: 11-28

ISSN: 0149-6395

Publisher: Taylor & Francis

Indexing

ISI Proceedings

ISI Web of Knowledge - 4 Citations

ISI Web of Science

Scopus - 3 Citations

COMPENDEX

Scientific classification

FOS: Engineering and technology > Materials engineering

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

Authenticus ID: P-004-4T1

DOI: 10.1080/01496390701747895

Abstract (EN): A gradient SMB model is used to analyze the separation performance of proteins in a step-wise salt gradient ion-exchange simulated moving bed (IE-SMB) for the nonlinear adsorption equilibrium isotherms. The strategy of the selection of salt gradient in IE-SMB chromatography is discussed based on the adsorption equilibrium isotherms of both BSA and myoglobin on Q-Sepharose FF anion exchanger in the pH 8 Tris buffer. The separation region is used to select the flow rates in the salt gradient IE-SMB chromatography for the separation of proteins. The separation region is calculated by the gradient SMB model where both the mass transfer resistance in the ion exchangers and axial liquid dispersion in columns are taken into account. Some strategies are suggested for the selections of salt gradient and flow rates in the IE-SMB chromatography with open loop configuration for two cases: (a) binary separation of proteins with the requirement of the complete recovery from extract stream and raffinate stream; and (b) protein purification from a stream with some impurities.

Language: English

Type (Professor's evaluation): Scientific