Development and evaluation of Pirkle-type chiral stationary phase for flash chromatography

• Type: Article in International Scientific Journal
• Date: 2022

Authors

Goncalves, L (Author) Other; Cravo, S (Author) Other; Carla Fernandes (Author) FFUP; Tiritan M.E. (Author) FFUP

Journal

Title: Journal of Chromatography A

Vol. 1675

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus

Other information

Authenticus ID: P-00W-MJG

DOI: 10.1016/j.chroma.2022.463156

Abstract (EN): Liquid chromatography is the most applied methodology for enantioseparation in preparative and semi-preparative scale; however, flash chromatography is seldom the first choice. This work proposes a new sustainable method to achieve pure enantiomers in mg scale. Herein, the functionalization of silica for flash chromatography columns with a suitable chiral selector, for subsequent quantitative enantiosepara-tion of chiral compounds, is described. Accordingly, the Whelk-O (R) 1 chiral selector was bonded to flash silica and packed into a reused solid phase extraction cartridge. For the evaluation of the enantioselective performance of the flash column, the enantiomers of a chiral derivative of xanthone were quantitatively enantioseparated with an average recovery of 70% and an enantiomer ratio (e.r.) of 99% and 97% for each enantiomer. Evaluation with the anti-inflammatory drug naproxen was also performed, resulting in an av-erage recovery of 95% and 89% and 95% e.r. for each enantiomer. The flash column showed high stability and load ability, versatility, and good reproducibility.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11