Xanthohumol Influences Preadipocyte Differentiation: Implication of Anti proliferative and Apoptotic Effects

• Type: Article in International Scientific Journal
• Date: 2008

Authors

mendes, v (Author) Other; monteiro, r (Author) FCNAUP; pestana, d (Author) Other; teixeira, d (Author) Other; calhau, c (Author) FMUP; azevedo, i (Author) Other

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 56 No. 24

Pages: 11631-11637

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 32 Citations

ISI Web of Science

Scopus - 39 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

CORDIS: Health sciences

Other information

Authenticus ID: P-003-T65

DOI: 10.1021/jf802233q

Abstract (EN): There is interest in the research of natural compounds that may interfere with the adipocyte life cycle, due to the growing prevalence of obesity and related complications. We aimed at studying the effect of xanthohumol (XN), a Humulus lupulus L. prenylflavonoid, on adipocytes measuring differentiation, proliferation, and apoptosis in 3T3-L1 cells. XN reduced differentiation, as revealed by decreased lipid content and peroxisome proliferator-activated receptor gamma expression, an effect more pronounced when cells were treated before or during differentiation induction. XN also decreased proliferation, as measured by sulforhodamine staining (IC(50) between 26 and 12 mu M for 24, 48, and 72 h), and preadipocyte Ki67 expression. Apoptosis was increased in preadipocytes and adipocytes. NF-kappa B activity was stimulated by XN in preadipocytes. Results suggest that XN may reduce adipocyte number, contributing to adipocyte hypertrophy. Taking into consideration the consequences of adipocyte hypertrophy, XN does not seem to improve the metabolic profile associated with obesity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7