Bovine serum albumin and myoglobin separation by size exclusion SMB

• Type: Article in International Scientific Journal
• Date: 2020

Authors

Rios, AG (Author) Other; Ana M. Ribeiro (Author) FEUP; Rodrigues, AE (Author) Other; Alexandre Ferreira (Author) FEUP

Journal

Title: Journal of Chromatography A

Vol. 1628

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 1 Citation

Other information

Authenticus ID: P-00S-K0S

DOI: 10.1016/j.chroma.2020.461431

Abstract (EN): The separation of the proteins Bovine Serum Albumin (BSA) and Myoglobin (Mb) was achieved by Size Exclusion Simulated Moving Bed (SE-SMB) and performed experimentally in the FlexSMB (R) unit, an SMB unit designed and built in the Laboratory of Separation and Reaction Engineering. Before accomplishing the separation experiments in the mentioned unit, separation regions were computed by simulation based on a phenomenological mathematical model to determine appropriate operating conditions. The developed model was validated in advance, against fixed-bed dynamic adsorption experimental results, for pure component and binary mixtures. Then the SMB experiments were carried out, and purities of the Mb on the extract and BSA on the raffinate streams were 98% and 96%, respectively. The achieved recoveries were 80% of Mb on the extract and 94% of BSA on the raffinate. Lastly, productivities of 6.4 g(protein) .l(ads)(-1) .day(-1) for the extract and 28.8 g(protein).l(ads)(-1) .day(-1) for the raffinate were obtained.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11