Resistance changes in Salmonella enterica serovar Typhimurium treated by High Hydrostatic Pressure and Pulsed Electric Fields and assessment of virulence changes by using Caenorhabditis elegans as a test organism

• Type: Article in International Scientific Journal
• Date: 2019

Authors

Sanz Puig, M (Author) Other; Velazquez Moreira, A (Author) Other; Torres, C (Author) Other; Guerrero Beltran, JA (Author) Other; Cunha, LM (Author) FCUP; Martinez, A (Author) Other; Rodrigo, D (Author) Other

Journal

Title: Innovative Food Science and Emerging Technologies

Vol. 51

Pages: 51-56

ISSN: 1466-8564

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 8 Citations

Other information

Authenticus ID: P-00N-W2R

DOI: 10.1016/j.ifset.2018.04.002

Abstract (EN): The goal of this study was to evaluate the development of Salmonella enterica subsp. enterica serovar Typhimurium resistance against Pulsed Electric Fields (PEF) and High Hydrostatic Pressure (HHP), and to study the possible virulence changes of the resistant subpopulations using Caenorhabditis elegans. Results showed that S. Typhimurium became resistant to both non-thermal technologies after four consecutive treatments. Survival probability of the worms (C. elegans) increased significantly when they were fed with S. Typhimurium treated by PEF four consecutive times in comparison with the other two subpopulations, untreated and treated once. For HHP, results indicated that up to percentile 50%, the survival probability of worms fed with treated microorganisms (once and four times) was greater than that of worms fed with untreated ones. Also, the C. elegans egg-laying pattern was modified and there were significant differences in the number of eggs laid in the first two days among the three subpopulations studied. Consequently, although S. Typhimurium develops microbial resistance against PEF or HHP treatments, when it is applied repeatedly, its virulence against C. elegans appears to decrease. Industrial relevance: Among the non-thermal technologies, Pulsed Electric Fields and High Hydrostatic Pressure have a great potential. Nevertheless, it is necessary to validate them from a safety point of view because of the huge amount of damaged cells that can be obtained if sublethal treatments are applied. These studies have industrial relevance in food safety ensuring proper processing when using these non-thermal technologies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6