Carbon dots prepared from citric acid and urea as fluorescent probes for hypochlorite and peroxynitrite

• Type: Article in International Scientific Journal
• Date: 2016

Authors

Simoes, EFC (Author) Other; Leitao, JMM (Author) Other; Joaquim C G E Esteves da Silva (Author) FCUP

Journal

Title: Mikrochimica Acta

Vol. 183

Pages: 1769-1777

ISSN: 0026-3672

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 105 Citations

Scopus - 108 Citations

Other information

Authenticus ID: P-00K-987

DOI: 10.1007/s00604-016-1807-6

Abstract (EN): Carbon dots (CDs) were prepared from citric acid and urea, and their fluorescence was found to be quenched by hypochlorite and peroxynitrite. Microwave based synthesis gives CDs with excitation/emission wavelength-dependent quantum yields (8 % at 400/520 nm; 10 % at 360/451 nm; 12 % at 350/420 nm). Quenching of fluorescence depends on pH values, and response is most selective and sensitive to hypochlorite at pH 4, and to peroxynitrite at pH 9. The lower detection limits are 0.5 and 1.5 mu M, respectively. The method was successfully applied to quantify hypochlorite and peroxynitrite in standard solutions and in spiked dilute serum samples.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9