Resveratrol and Grape Extract-loaded Solid Lipid Nanoparticles for the Treatment of Alzheimer's Disease

• Type: Article in International Scientific Journal
• Date: 2017

Authors

Joana A. Loureiro (Author) FEUP; Stephanie Andrade (Author) FEUP; Ana Duarte (Author) Other; Ana Rute Neves (Author) Other; Joana Fontes Queiroz (Author) Other; Claúdia Nunes (Author) Other; Emmanuel Sevin (Author) Other; Laurence Fenart (Author) Other; Fabien Gosselet (Author) Other; Manuel A. N. Coelho (Author) FEUP; Maria do Carmo Pereira (Author) FEUP

Journal

Title: Molecules

Vol. 22 No. 2

Pages: 1-16

ISSN: 1420-3049

Publisher: MDPI

Indexing

ISI Web of Knowledge - 67 Citations

Scopus - 108 Citations

Associated Projects

PTDC/QUI-BIQ/118076/2010 - Liposomas funcionalizados com anticorpos para a doença de Alzheimer

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPAE/CEFT - RL2 - (Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health

Other information

Authenticus ID: P-00M-JZ9

DOI: 10.3390/molecules22020277

Abstract (EN): The aggregation of amyloid- peptide (A) has been linked to the formation of neuritic plaques, which are pathological hallmarks of Alzheimer's disease (AD). Various natural compounds have been suggested as therapeutics for AD. Among these compounds, resveratrol has aroused great interest due to its neuroprotective characteristics. Here, we provide evidence that grape skin and grape seed extracts increase the inhibition effect on A aggregation. However, after intravenous injection, resveratrol is rapidly metabolized into both glucuronic acid and sulfate conjugations of the phenolic groups in the liver and intestinal epithelial cells (within less than 2 h), which are then eliminated. In the present study, we show that solid lipid nanoparticles (SLNs) functionalized with an antibody, the anti-transferrin receptor monoclonal antibody (OX26 mAb), can work as a possible carrier to transport the extract to target the brain. Experiments on human brain-like endothelial cells show that the cellular uptake of the OX26 SLNs is substantially more efficient than that of normal SLNs and SLNs functionalized with an unspecific antibody. As a consequence, the transcytosis ability of these different SLNs is higher when functionalized with OX-26.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 16