Microbiology

Code:

CN21003

Acronym:

MICROB

Keywords
Classification	Keyword
OFICIAL	Health Sciences
OFICIAL	Natural Sciences

Instance: 2013/2014 - 1S

Active?	Yes
Web Page:	http://moodle.up.pt/
Course/CS Responsible:	Nutrition Sciences

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
CNUP	75	Plano oficial	2	-	5,5	56	148,5

Teaching language

Portuguese

Objectives

The main objective of this first discipline of microbiology is to understand the importance of the different areas of microbiology and their applications within the context of Nutrition Sciences.
This discipline intended to present the fundamental principles of Microbiology in view of the relationship between the main groups of micro-organisms (bacteria, fungi, parasites and viruses) and man. The theoretical program addresses the general characteristics of various microorganisms (bacteria, fungi, parasites and viruses) and its relationship with the human host as etiological agents of infection. The laboratory program aims to make known to students the basic methodology of the study of microbiology and encouraging their interconnection with the theoretical concepts of microbiology.

Learning outcomes and competences

Students should be able to:

- Know and recognize the general characteristics of various microorganisms (bacteria, fungi, parasites and virus) and their relationship with man as etiologic agents of disease.

- Understand the importance / impact of various groups of microorganisms (bacteria, fungi, parasites and virus) to humans, mainly of human microbiome.

- Know the factors that affect microbial growth and antimicrobial control methods.

- Identify and understand the fundamental concepts of the different phenotypic and genotypic methods used in the identification and typing of microorganisms.

- Know how to analyze, interpret and communicate the results of different phenotypic and genotypic methods used in the identification and typing of microorganisms.

Working method

Presencial

Program

Theoretical classes

- Introduction to the study of microbiology. Taxonomy of living world. Major groups of microrganisms: bacteria, fungi, parasites and viruses.
- Bacteria: anatomy, physiology and bacterial genetics. Examples.
- Fungi: Morphology, metabolism and growth. Mechanisms of reproduction. Examples. 
- Parasites: General characteristics of of protozoa and metazoa. Taxonomy and nomenclature. Examples.
- Virus: General properties and morphology. Classification. Examples.
- Microbiological control. Antimicrobial agents. Evolution of resistance to antimicrobial agents.
- Identification and typing of microorganisms. Taxonomy. Phenotypic and genotypic methods. 

- The relationship between humans and microorganisms. Normal microbial flora of the human body. Microorganismos as etiologic agents of disease. Virulence factors of pathogenic microorganisms and host defenses.  

Practical classes

- Introduction to laboratory practice. Safety standards in the microbiology laboratory. Basic tools of a microbiology laboratory. Aseptic technique.
- General concepts on microscopy. Light microscopy and bacterial morphology. Preparation of microorganisms for optical microscope. Living bacterial preparations. Staining techniques: simple staining and differential staining: Gram stain, Ziehl Neelsen stain, endospore and capsule staining.
- Cultivation techniques. Microbiological culture media in bacteriology. Plating procedures for isolation, counting and identification.
- Antimicrobial susceptibility tests.
- Identification of microorganisms: biochemical tests and alternative microbiological methods. Bioinformatic tools in microbiology.

Mandatory literature

Ferreira Wanda F. Canas 340; Microbiologia. ISBN: 978-972-757-515-2
Willey Joanne M.; Prescottc2b4s Microbiology. ISBN: 978-007-131367-4
Harley John P.; Laboratory exercises in microbiology. ISBN: 978-0-07-729281-2
Forbes Betty A.; Bailey & Scottc2b4s diagnostic microbiology. ISBN: 0-323-01678-2
Madigan Michael T. 070; Brock biology of microorganisms. ISBN: 978-0321-53615-0

Teaching methods and learning activities

Theoretical classes (2 hours / week)
The lectures are organized by themes, looking up through the expositive method, with informatic support (e.g PowerPoint presentations), providing to students the basic concepts of Microbiology, encouraging their interconnection with the laboratory classes.

Laboratory classes (2 hours / week)
The practical classes arise in a laboratory with the aim of acquainting students with the basic techniques of microbiology, encouraging discussion of the results. The laboratory work will be developed individually or in groups, and may be accompanied by written work or oral presentations.

We encourage the active participation of students in the teaching / learning, the development of scientific thinking, including experimental design, recording and reporting of results and fundamental concepts of microbiology in a written or oral format.

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Designation	Weight (%)
Exame	75,00
Trabalho laboratorial	25,00
Total:	100,00

Eligibility for exams

The taking of attendance in the discipline is subject to the presence of a student in 75% of all practical classes, according to the Academic Regulation of FCNAUP, and students need to obtain at least 9.50 values in the distribution assessment (AD).

Students whose status allows dispensing of laboratory classes should contact the teacher responsible for the course in the first weeks of classes. In these cases, approval will be obtained in the distributed assessment (AD) by performing work that can be done outside the classroom, laboratory and will be disclosed and guided by the teacher of the course. Approval in the distributed assessment (AD) is a prerequisite for admission to the student's final exam (written test).

Calculation formula of final grade

The evaluation in the UC of Microbiology is based on distributed assessment (AD) and a final exam (EF), under the following conditions:

a) The distributed assessment (AD) is the continuous assessment in the classroom practices: preparation and implementation of laboratory work, discussion of results, direct observation of the participation and interest demonstrated, papers in written and/or oral format. The note is posted before of the written test.

a) The final exam (EF) is a written test, which is performed in the final exam period of assessment proposed by the FCNAUP. Are approved students who obtain the written test a rating equal to or greater than 9.50 values. Final Exam includes response to a set of issues for development that may be of multiple choice answer, sentence completion, true or false, or limited response.

c) The final classification (CF) is the result of: CF = (EF x 75%) + (AD x 25%)

Special assessment (TE, DA, ...)

Students whose status allows dispensing of laboratory classes should contact the teacher responsible for the course in the first weeks of classes. In these cases, approval will be obtained in the distributed assessment (AD) by performing work that can be done outside the classroom, laboratory and will be disclosed and guided by the teacher of the course. Approval in the distributed assessment (AD) is a prerequisite for admission to the student's final exam (written test).

 

Classification improvement

According to the Academic Regulation of FCNAUP.