Application of response surface methodology for obtaining lettuce (Lactuca sativa L) by-products extracts with high antioxidative properties

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Teresa Gomes (Author) Other; Teresa Delgado (Author) Other; Anabela Ferreira (Author) Other; Jose Alberto Pereira (Author) Other; Paula Baptista (Author) Other; Susana Casal (Author) FFUP; Elsa Ramalhosa (Author) Other

Journal

Title: Industrial Crops and Products

Vol. 44

Pages: 622-629

ISSN: 0926-6690

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 13 Citations

Scientific classification

FOS: Agrarian Sciences > Agricultural biotechnology

Other information

Authenticus ID: P-002-1CV

DOI: 10.1016/j.indcrop.2012.09.011

Abstract (EN): The main objective of the present work was to optimize the extraction conditions for simultaneous maximization of total reducing (TRC) and antioxidant (AC) capacities for lettuce (Lactuca sativa L.) by-products extracts, using response surface methodology. For this, a design of experiments (DOE) with different combinations of solvents (water, methanol and acetone) extraction temperatures (30-60 degrees C) and time (10-60 min) on the TRC and AC was applied. Higher and consistent fittings using second order polynomial models of the experimental data with regard to TRC (R-2 = 0.529 P-lack of fit > 0.05) and AC (R-2 = 0.900, P-lack of fit > 0.05) were obtained with methanol. The optimum extraction conditions based on combination responses for TRC and AC were: 30% methanol (v/v), 60 degrees C and 60 min. A close agreement between experimental and predicted values was found when applying these conditions. Furthermore, when aqueous extracts were prepared (e.g. 45 degrees C, 10 min), these presented similar TRC and AC properties to those obtained by the above optimum extraction conditions, having the advantage of applying mild extraction conditions and avoiding the use of organic solvents in their preparation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8