Successful application of the DiversiLab repetitive-sequence-based PCR typing system for confirmation of the circulation of a multiresistant Pseudomonas aeruginosa clone in different hospital wards

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Csilla Ratkai (Author) Other; Luisa V Peixe (Author) FFUP; Filipa Grosso (Author) Other; Ana R Freitas (Author) Other; Patricia Antunes (Author) FCNAUP; Eleonora Fodor (Author) Other; Edit Hajdu (Author) Other; Elisabeth Nagy (Author) Other

Journal

Title: Diagnostic Microbiology and Infectious Disease

Vol. 67 No. 2

Pages: 202-206

ISSN: 0732-8893

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

ISI Web of Science

Scopus - 16 Citations

Scientific classification

CORDIS: Health sciences

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-003-5Y7

DOI: 10.1016/j.diagmicrobio.2010.01.010

Abstract (EN): The applicability of the repetitive-sequence-based PCR (rep-PCR) based DiversiLab system was tested compared with the pulsed field gel electrophoresis (PFGE) to type a phenotypically similar subset of a large collection of multiresistant Pseudomonas aeruginosa strains isolated during a 17-month period from patients treated in different wards including 4 intensive care units (ICUs). Five environmental P. aeruginosa isolates obtained from one of the ICUs were also included. The DiversiLab system and the PFGE demonstrated the genetic relationship among the isolates with the same efficacy. One of the environmental isolates had the same rep-PCR type as the circulating clone. Multilocus sequence typing of one of the clinical isolates of the circulating clone proved that it is a member of a clonal complex of P. aeruginosa that has not been previously described in clinical samples.

Language: English

Type (Professor's evaluation): Scientific

Notes: <a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=WOS&DestLinkTy pe=FullRecord&KeyUT=000278194700014">Indexado na ISI Web of Science</a>

No. of pages: 5