Impact of modified diamond-like carbon coatings on the spatial organization and disinfection of mixed-biofilms composed of Escherichia coli and Pantoea agglomerans industrial isolates

• Type: Article in International Scientific Journal
• Date: 2018

Authors

Luciana Gomes (Author) FEUP; J. Deschamps (Author) Other; R. Briandet (Author) Other; Filipe J. Mergulhão (Author) FEUP

Journal

Title: International Journal of Food Microbiology

Vol. 277

Pages: 74-82

ISSN: 0168-1605

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 2 Citations

Scopus - 22 Citations

INSPEC

Associated Projects

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

SUSCLEAN - Sustainable Cleaning and Disinfection in Fresh-Cut Food Industries

Other information

Authenticus ID: P-00N-W6W

DOI: 10.1016/j.ijfoodmicro.2018.04.017

Abstract (EN): This work investigated the effects of diamond-like carbon (DLC) coatings on the architecture and biocide reactivity of dual-species biofilms mimicking food processing contaminants. Biofilms were grown using industrial isolates of Escherichia coli and Pantoea agglomerans on bare stainless steel (SST) and on two DLC surface coatings (a-C:H:Si:O designated by SICON (R) and a-C:H:Si designated by SICAN) in order to evaluate their antifouling activities. Quantification and spatial organization in single- and dual-species biofilms were examined by confocal laser scanning microscopy (CLSM) using a strain specific labelling procedure. Those assays revealed that the E. cob isolate exhibited a higher adhesion to the modified surfaces and a decreased susceptibility to disinfectant in presence of P. agglomerans than alone in axenic culture. While SICON (R) reduced the short-term growth of E. con in axenic conditions, both DLC surfaces increased the E. coli colonization in presence of P. agglomerans. However, both modified surfaces triggered a significantly higher log reduction of E. coli cells within mixed-species biofilms, thus the use of SICON (R) and SICAN surfaces may be a good approach to facilitate the disinfection process in critical areas of food processing plants. This study presents a new illustration of the importance of interspecies interactions in surface-associated community functions, and of the need to evaluate the effectiveness of hygienic strategies with relevant multi-species consortia.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9