Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Almeida, MJ (Author) Other; Pereira, L (Author) Other; Milet, C (Author) Other; Haigle, J (Author) Other; Mário Adolfo Barbosa (Author) FEUP; Lopez, E (Author) Other

Journal

Title: Journal of Biomedical Materials Research

Vol. 57

Pages: 306-312

ISSN: 0021-9304

Publisher: Heterocorporation

Indexing

ISI Web of Knowledge - 26 Citations

Scopus - 28 Citations

Other information

Authenticus ID: P-000-SWR

DOI: 10.1002/1097-4636(200111)57:2<306::aid-jbm1172>3.0.co;2-h

Abstract (EN): In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal luna. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell laver of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 mug protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. (C) 2001 John Wiley & Sons, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7