Poly-N-Acetylglucosamine Production by Staphylococcus epidermidis Cells Increases Their In Vivo Proinflammatory Effect

• Type: Article in International Scientific Journal
• Date: 2016-09-19

Authors

Pedro Ferreirinha (Author) ICBAS; Begoña Pérez-Cabezas (Author) Other; Bruna Myiazawa (Author) Other; Angela França (Author) Other; Virgínia Carvalhais (Author) Other; Augusto Faustino (Author) ICBAS; Anabela Cordeiro-da-Silva (Author) FFUP; Luzia Teixeira (Author) ICBAS; Gerald B. Pier (Author) Other; Nuno Cerca (Author) Other; Manuel Vilanova (Author) ICBAS; Alexandra Correia (Author) ICBAS

Journal

Title: Infection and Immunity

Vol. 84

Pages: 2933-2943

ISSN: 0019-9567

Publisher: American Society for Microbiology

Indexing

ISI Web of Knowledge - 7 Citations

ISI Web of Science

Scopus - 8 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Basic medicine

CORDIS: Health sciences > Medical sciences > Medicine > Immunology

Other information

Authenticus ID: P-00M-3ZN

DOI: 10.1128/iai.00290-16

Abstract (EN): Poly-N-acetylglucosamine (PNAG) is a major component of the Staphylococcus epidermidis biofilm extracellular matrix. However, it is not yet clear how this polysaccharide impacts the host immune response and infection-associated pathology. Faster neutrophil recruitment and bacterial clearance were observed in mice challenged intraperitoneally with S. epidermidis biofilm cells of the PNAG-producing 9142 strain than in mice similarly challenged with the isogenic PNAG-defective M 10 mutant. Moreover, intraperitoneal priming with 9142 cells exacerbated liver inflammatory pathology induced by a subsequent intravenous S. epidermidis challenge, compared to priming with M 10 cells. The 9142-primed mice had elevated splenic CD4(+)T cells producing gamma interferon and interleukin-17A, indicating that PNAG promoted cell-mediated immunity. Curiously, despite having more marked liver tissue pathology, 9142-primed mice also had splenic T regulatory cells with greater suppressive activity than those of their M 10-primed counterparts. By showing that PNAG production by S. epidermidis biofilm cells exacerbates host inflammatory pathology, these results together suggest that this polysaccharide contributes to the clinical features associated with biofilm-derived infections.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11