Simultaneous determination of reduced and oxidized glutathione in freshly isolated rat hepatocytes and cardiomyocytes by HPLC with electrochemical detection

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Fernando Remiï¿¿o (Author) FFUP; Helena Carmo (Author) Other; Fï¿¿lix Carvalho (Author) FFUP; Maria L Bastos (Author) FFUP

Journal

Title: Biomedical Chromatography

Vol. 14

Pages: 468-473

ISSN: 0269-3879

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-00G-XNJ

DOI: 10.1002/1099-0801(200011)14:7<468::aid-bmc10>3.0.co;2-c

Abstract (EN): Glutathione and glutathione disulphide constitute an essential thiol redox system present in the cell. The balance in favour of the latter is an indication of oxidative stress. Glutathione and glutathione disulphide quantification in isolated cells may therefore be essential for the evaluation of mechanistic and comparative studies of toxic xenobiotics. In this study, a rapid and sensitive isocratic reverse-phase high-performance liquid chromatographic method using coulometric detection was implemented for the simultaneous detection of glutathione and glutathione disulphide, in freshly isolated hepatocytes and cardiomyocytes of the rat. The method implemented proved to be effective for the measurement of glutathione and glutathione disulphide in control conditions and for the detection of variations in this redox system, induced by tert-butylhydroperoxide. tert-Butylhydroperoxide is an organic peroxide, which has been used as a model molecule for inducing oxidative stress in isolated cells. A comparative study with a previously published HPLC-electrochemical detection method was performed. Copyright (C) 2000 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6