The development and evaluation of an HPLC-DAD method for the analysis of the phenolic fractions from in vivo and in vitro biomass of Hypericum species

• Type: Article in International Scientific Journal
• Date: 1999

Authors

Dias, ACP (Author) Other; Seabra, RM (Author) Other; Andrade, PB (Author) FFUP; Fernandes Ferreira, M (Author) Other

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 22

Pages: 215-227

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 55 Citations

Scopus - 56 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-5WS

DOI: 10.1081/jlc-100101655

Abstract (EN): A new HPLC-DAD method for separation and identification of the main phenolics present in in vivo and in vitro biomass of Hypericum perforatum and Hypericum androsaemum has been developed. This method accomplished the direct identification of 22 compounds including flavonoids, hypericins, phloroglucinols, and phenolic acids. The HPLC profiles obtained in the course of this work clearly evidenced a distinct phenolic production between in vivo and in vitro biomass. For example calli and suspended cells produced mainly xanthones while in in vivo plants of both species these compounds were not detected. Some luteolin flavone type compounds were identified in calli and suspended cells of H. perforatum while quercetin was found in suspended cells of H. androsaemum. Flavonoids, namely those related to quercetin, were the major metabolites in methanolic extracts from in vivo plants. Hypericins were detected in in vivo plants and in in vitro shoots of H. perforatum but not in calli or suspended cells of the same species. Aurones, produced by in vitro cultures were not detected in in vivo plants of H. perforatum.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 13