NO Fluorescence Quantification by Chitosan CdSe Quantum Dots Nanocomposites

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Eliana F C Simoes (Author) Other; Joao M M Leitao (Author) Other; Joaquim C G E Esteves da Silva (Author) FCUP

Journal

Title: Journal of Fluorescence

Vol. 24

Pages: 639-648

ISSN: 1053-0509

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 9 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-008-XFN

DOI: 10.1007/s10895-013-1337-0

Abstract (EN): The quantification of nitric oxide (NO) based on the quenching of the fluorescence of a nanocomposites sensor constituted by cadmium/selenium quantum dots (CdSe) stabilized by chitosan (CS) and mercaptosuccinic acid (MSA) is assessed. The optimization of the response of the CS-CdSe-MSA nanocomposites to NO was done by multivariate response surface experimental design methodologies. The highest fluorescence quenching was obtained at pH 5.5 and at room temperature. The NO quantification capability of CS-CdSe-MSA was evaluated using standard solutions and a NO donor reagent. A large linear working range from 5 to 200 mu M and a limit of detection of 1.86 mu M were obtained. Better quantification results were obtained using the NO donor reagent. Besides NO, the response of the fluorescence of CS-CdSe-MSA to the main reactive oxygen and nitrogen species and similar NO compounds was also assessed.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10