pH opposite effects on synthesis of dinucleoside polyphosphates and on oxidation reactions catalyzed by firefly luciferase

• Type: Article in International Scientific Journal
• Date: 2003

Authors

fraga, h (Author) Other; da silva, jcge (Author) FCUP; fontes, r (Author) FMUP

Journal

Title: FEBS Letters

Vol. 543

Pages: 37-41

ISSN: 0014-5793

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 17 Citations

Scopus - 17 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-GM0

DOI: 10.1016/s0014-5793(03)00382-x

Abstract (EN): Previous results have shown that an oxidizing product of firefly luciferin, dehydroluciferyl-adenylate, is the main intermediate in the process of synthesis of dinucleoside polyphosphates catalyzed by firefly luciferase (EC 1.13.12.7). However, we have found that the pH effects on the luciferase oxidizing processes and on the synthesis of dinucleoside polyphosphate are opposite: acidic assay media enhance the synthesis of dinucleoside polyphosphate and inhibit the oxidizing processes. The reason for this apparent contradiction lies on the activation effect of low pH on the adenylate transfer reaction from dehydroluciferyl-adenylate to the acceptor nucleotide. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5