Antioxidant and antimutagenic activity of N-(2-carboxyethyl)chitosan

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Kogan, G (Author) Other; Skorik, YA (Author) Other; Zitnanova, I (Author) Other; Krizkova, L (Author) Other; Durackova, Z (Author) Other; Gomes, CAR (Author) FCUP; Yatluk, YG (Author) Other; Krajcovic, J (Author) Other

Journal

Title: Toxicology and Applied Pharmacology

Vol. 201

Pages: 303-310

ISSN: 0041-008X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 72 Citations

Scopus - 84 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-7AK

DOI: 10.1016/j.taap.2004.05.009

Abstract (EN): The antioxidant and antimutagenic activities of the novel carboxyethyl derivatives of chitosan with three different degrees of substitution have been assayed in vitro in the unicellular flagellate Euglena gracilis subjected to the action of genotoxic agents acridine orange and ofloxacin. It has been demonstrated that chitosan derivatives exhibit concentration-dependent protective antigenotoxic activity against both mutagens. It is suggested that different mechanisms may be involved in its protective action-antioxidant activity in case of ofloxacin-induced DNA damage, as well as possible interaction with the cell membrane that prevents acridine orange from reaching the genetic compartments and subsequent damaging DNA through intercalative binding. Direct adsorption of acridine orange on chitosan derivatives was ruled out as a possible mechanism of protection on the basis of spectrophotometric measurements. Dependence of the antimutagenic proper-ties of the studied chitosan derivatives on the degree of substitution was reversed in experiments involving acridine orange and ofloxacin. which also indicated different mechanisms of protection involved in these two cases.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8