Validation of a fast and accurate chromatographic method for detailed quantification of vitamin E in green leafy vegetables

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Rebeca Cruz (Author) Other; Susana Casal (Author) FFUP

Journal

Title: Food Chemistry

Vol. 141 No. 14

Pages: 1175-1180

ISSN: 0308-8146

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 21 Citations

Scopus - 23 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-006-68X

DOI: 10.1016/j.foodchem.2013.03.099

Abstract (EN): Vitamin E analysis in green vegetables is performed by an array of different methods, making it difficult to compare published data or choosing the adequate one for a particular sample. Aiming to achieve a consistent method with wide applicability, the current study reports the development and validation of a fast micro-method for quantification of vitamin E in green leafy vegetables. The methodology uses solid-liquid extraction based on the Folch method, with tocol as internal standard, and normal-phase HPLC with fluorescence detection. A large linear working range was confirmed, being highly reproducible, with inter-day precisions below 5% (RSD). Method sensitivity was established (below 0.02 mu g/g fresh weight), and accuracy was assessed by recovery tests (>96%). The method was tested in different green leafy vegetables, evidencing diverse tocochromanol profiles, with variable ratios and amounts of alpha- and gamma-tocopherol, and other minor compounds. The methodology is adequate for routine analyses, with a reduced chromatographic run (<7 min) and organic solvent consumption, and requires only standard chromatographic equipment available in most laboratories.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6