Optimized conditions and analytical performance for the determination of Cu in serum and urine samples using a single GFAAS procedure

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Almeida, AA (Author) FFUP; Lima, JLFC (Author) FFUP

Journal

Title: Atomic Spectroscopy

Vol. 22

Pages: 324-330

ISSN: 0195-5373

Publisher: Perkin-Elmer Corp.

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 15 Citations

Scientific classification

FOS: Natural sciences > Physical sciences

Other information

Authenticus ID: P-000-VJV

Abstract (EN): The determination of copper in biological samples is of major importance in clinical chemistry and very often, especially when Wilson's disease is suspected, the simultaneous analysis of both serum and urine is necessary. Therefore, a single analytical procedure with proven reliability in the simultaneous determination of copper in both matrices becomes of great practical interest for clinical laboratories. This work describes the optimization and evaluation of a Zeeman-effect background correction GFAAS method. It differs only in the prior dilution of the serum samples (1+24) and allows the determination of copper in both serum and urine samples with proven accuracy, precision, and robustness. The method provides a limit of detection of 0.98 mug/L, a limit of quantification of 3.3 mug/L, and a characteristic mass of about 23 pg. Repeatability (%RSD) was 1.3% for a standard solution (20 mug/L), 2.4% for urine (26 mug/L), and 1.4% for serum (29 mug/L) samples. Between-run and between-day reproducibility was typically better than 5%. Accuracy has been assessed using the standard additions method/calibration curves slope ratio, which was 1.057 for serum and 1.023 for urine, and by the analysis of commercial control samples. Additional evaluation of the results obtained in the determination of copper in serum was made by comparison with Flame AAS analysis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7