Validation of a Micromethod for Quantification of Lutein and β-Carotene in Olive Oil

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Lúcia Maia (Author) Other; Susana Casal (Author) FFUP; M. Beatriz P. P. Oliveira (Author) FFUP

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 31 No. 5

Pages: 733-742

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Science

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences

Other information

Resumo (PT): A micromethod was developed and validated for the quantification of lutein and β-carotene in olive oils. The compounds are liquid-liquid extracted with n-hexane and n,n-dimethylformamide (DMF), by vortex mixing and centrifugation. The n-hexane phase retains β-carotene, whereas the DMF phase retains lutein. This last fraction is concentrated and analyzed by RP-HPLC/diode-array, with a gradient of acetonitrile, water, and ethyl acetate, using β-apo-8'-carotenal as I.S. β-carotene is quantified by UV/Vis measurements at 454 nm, by the external standard method. The method shows appropriate inter- and intra-day reproducibility, accuracy, and linearity for both compounds, allowing fast analysis of several samples simultaneously, with a relatively small consumption of organic solvents. The analytical methodology was applied to several samples of commercial Portuguese PDO olive oils. <br> <br> Keywords: Olive oil; RP-HPLC; Lutein; β-Carotene; Internal standard; Micromethod <br> <a target="_blank" href="http://web.ebscohost.com/ehost/detail?vid=4&hid=103&sid=aed51d49-d746-43fd-8e5b-150fd7bc5820%40sessionmgr109"> Texto integral </a> <br> <br>

Abstract (EN): A micromethod was developed and validated for the quantification of lutein and β-carotene in olive oils. The compounds are liquid-liquid extracted with n-hexane and n,n-dimethylformamide (DMF), by vortex mixing and centrifugation. The n-hexane phase retains β-carotene, whereas the DMF phase retains lutein. This last fraction is concentrated and analyzed by RP-HPLC/diode-array, with a gradient of acetonitrile, water, and ethyl acetate, using β-apo-8'-carotenal as I.S. β-carotene is quantified by UV/Vis measurements at 454 nm, by the external standard method. The method shows appropriate inter- and intra-day reproducibility, accuracy, and linearity for both compounds, allowing fast analysis of several samples simultaneously, with a relatively small consumption of organic solvents. The analytical methodology was applied to several samples of commercial Portuguese PDO olive oils. <br> <br> Keywords: Olive oil; RP-HPLC; Lutein; β-Carotene; Internal standard; Micromethod <br> <a target="_blank" href="http://web.ebscohost.com/ehost/detail?vid=4&hid=103&sid=aed51d49-d746-43fd-8e5b-150fd7bc5820%40sessionmgr109"> Full text </a> <br> <br>

Language: Portuguese

Type (Professor's evaluation): Scientific