Development and Validation of a Matrix Solid-Phase Dispersion Method to Determine Acrylamide in Coffee and Coffee Substitutes

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Cristina M D Dias Soares (Author) Other; Rita C Alves (Author) Other; Susana Casal (Author) FFUP; Beatriz B P P Oliveira (Author) FFUP; Jose Oliveira Fernandes (Author) FFUP

Journal

Title: Journal of Food Science

Vol. 75

Pages: T57-T63

ISSN: 0022-1147

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 35 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-003-7X6

DOI: 10.1111/j.1750-3841.2010.01545.x

Abstract (EN): The present study describes the development and validation of a new method based on a matrix solid-phase dispersion (MSPD) sample preparation procedure followed by GC-MS for determination of acrylamide levels in coffee (ground coffee and brewed coffee) and coffee substitute samples. Samples were dispersed in C(18) sorbent and the mixture was further packed into a preconditioned custom-made ISOLUTE bilayered SPE column (C(18)/Multimode; 1 g + 1 g). Acrylamide was subsequently eluted with water, and then derivatized with bromine and quantified by GC-MS in SIM mode. The MSPD/GC-MS method presented a LOD of 5 mu g/ kg and a LOQ of 10 mu g/kg. Intra and interday precisions ranged from 2% to 4% and 4% to 10%, respectively. To evaluate the performance of the method, 11 samples of ground and brewed coffee and coffee substitutes were simultaneously analyzed by the developed method and also by a previously validated method based in a liquid-extraction (LE) procedure, and the results were compared showing a high correlation between them.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7