Experimental characterization and modelling of analytical monolithic column

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Zabka, M (Author) Other; Minceva, M (Author) Other; Alírio Rodrigues (Author) FEUP

Journal

Title: Journal of Biochemical and Biophysical Methods

Vol. 70 No. 1

Pages: 95-105

ISSN: 0165-022X

Publisher: Elsevier B.V.

Indexing

ISI Proceedings

ISI Web of Knowledge - 15 Citations

Scopus - 13 Citations

Scientific classification

CORDIS: Technological sciences > Engineering > Chemical engineering

Other information

Authenticus ID: P-004-BRX

DOI: 10.1016/j.jbbm.2006.10.002

Resumo (PT): PHASE LIQUID-CHROMATOGRAPHY; SIZE-EXCLUSION CHROMATOGRAPHY; POROUS SILICA COLUMNS; STATIONARY PHASES; BAND PROFILES; ENANTIOMER SEPARATIONS; PROTEIN SEPARATION; BASIC COMPOUNDS; PORE STRUCTURE; PRESSURE-DROP

Abstract (EN): Hydrodynamics, equilibrium and kinetics of adsorption in a silica-based monolithic column Chromolith Performance RP-18e (Merck KgaA, Germany) have been studied. The column permeability was calculated according to the Darcy law for laminar flow. The efficiency of the monolithic column was characterized through the height equivalent to a theoretical plate (HETP) for myoglobin, phenol and progesterone. The 2-D single channel mathematical model has been applied to describe the adsorption dynamics. Parabolic velocity profile, axial and radial diffusion in the monolith channel, linear driving force model for the mass transfer in the monolith channel skeleton wall and linear adsorption equilibrium were assumed. The mathematical model gives good prediction of the experimental elution peaks.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 11