Silver nanoparticles obtained from Brazilian pepper extracts with synergistic anti-microbial effect: production, characterization, hydrogel formulation, cell viability, and in vitro efficacy

• Type: Article in International Scientific Journal
• Date: 2021

Authors

de Oliveira, DM (Author) Other; Menezes, DB (Author) Other; Andrade, LR (Author) Other; Lima, FD (Author) Other; Hollanda, L (Author) Other; Zielinska, A (Author) Other; Sanchez Lopez, E (Author) Other; Souto, EB (Author) Other; Severino, P (Author) Other

Journal

Title: Pharmaceutical Development and Technology

Vol. 26

Pages: 539-548

ISSN: 1083-7450

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 0 Citations

Scopus - 2 Citations

Other information

Authenticus ID: P-00T-PQ6

DOI: 10.1080/10837450.2021.1898634

Abstract (EN): The synthesis of silver nanoparticles using plant extracts is known as a green approach, as it does not require the use of high pressure, energy, high temperature, or toxic chemicals. The approach makes use of plant extracts in a process called bioreduction, which is mediated by enzymes, proteins, amino acids, and metabolites found in bark, seed, and leaf extracts, transforming silver ions into metallic silver. This work aimed at developing silver nanoparticles (AgNPs) from Brazilian pepper, applying this green methodology. Hydroalcoholic extract of leaves of Schinus terebinthifolius Raddi was prepared and its concentration of polyphenols, tannins, and saponins quantified. The produced nanoparticles were characterized by UV-Vis spectroscopy, thermogravimetric analysis (TG), dynamic light scattering (DLS), and zeta potential (ZP). AgNPs were formulated in sodium alginate hydrogels to obtain a nano-based semi-solid formulation for skin application. The obtained silver nanoparticles of mean size between 350 and 450 nm showed no cytotoxicity against L929 mouse fibroblasts within the concentration range of 0.025 mg/mL and 10 mg/mL. Schinus terebinthifolius Raddi was found to enhance microbial inhibition against the tested strains, especially against gram-negative bacteria. Its potential use as an alternative to overcome bacterial resistance can be expected.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10